Health Research Alliance
CIRCULATIONAHA.122.059346.pdf (3.87 MB)
Download file

RNA-binding Protein LIN28a Regulates New Myocyte Formation in the Heart via lncRNA-H19

Download (3.87 MB)
posted on 2023-01-16, 18:42 authored by Vagner Oliveira-Carvalho Rigaud, Robert C. Hoy, Justin Kurian, Clare Zarka, Michael Behanan, Isabella Brosious, Jennifer Pennise, Tej M. Patel, Tao Wang, Jaslyn Johnson, Lindsay Kraus, Sadia Mohsin, Steven R. Houser, Mohsin Khan

 Background: Developmental cardiac tissue holds remarkable capacity to regenerate after injury and consists of regenerative mononuclear and diploid cardiomyocytes (MNDCMs). Upon maturation, MNDCMs become binucleated or polyploid and exit the cell cycle. Interestingly, cardiomyocyte (CM) metabolism undergoes a profound shift that coincides with cessation of regeneration in the postnatal heart. However, whether reprogramming metabolism promotes persistence of regenerative MNDCMs enhancing cardiac function and repair after injury is unknown. Here, we identify a novel role for RNA-binding protein LIN28a, a master regulator of cellular metabolism, in cardiac repair following injury. Methods: LIN28a overexpression was tested using mouse transgenesis on postnatal CM numbers, cell cycle and response to apical resection (AR) injury. Using, neonatal and adult cell culture system and adult and MADM myocardial injury models in mice, effect of LIN28a overexpression on cardiomyocyte cell cycle and metabolism was tested. Finally, isolated adult CMs from LIN28a and wildtype mice 4 days after myocardial injury, were used for RNA-immunoprecipitation sequencing (RIP-seq). Results: LIN28a was found as primarily active during cardiac development and rapidly decreases after birth. LIN28a reintroduction at P1, P3, P5, and P7 decreased maturation-associated polyploidization, nucleation, and cell size, enhancing CM cell cycle activity in LIN28a transgenic pups compared to WT littermates. Moreover, LIN28a overexpression extended CM cell cycle activity beyond P7 concurrent with increased cardiac function 30 days after AR. In the adult heart, LIN28a overexpression attenuated CM apoptosis, enhanced cell cycle activity, cardiac function, and survival in mice 12 weeks after myocardial infarction compared to WT littermate controls. Alternatively, LIN28a small molecule inhibitor attenuated pro-reparative effects of LIN28a on the heart. Mechanistically, Neonatal rat ventricular myocytes (NRVMs) overexpressing LIN28a showed increased glycolysis, ATP production and levels of metabolic enzymes compared to control. LIN28a immunoprecipitation followed by RNA sequencing (RIPseq) in CMs isolated from LIN28a-overexpressing hearts after injury identified lncRNA-H19 as its most significantly altered target. Ablation of lncRNA-H19 blunted LIN28a-induced enhancement on CM metabolism and cell cycle activity. Conclusions: Collectively, LIN28a reprograms CM metabolism and promotes persistence of MNDCMs in the injured heart enhancing pro-reparative processes thereby linking CM metabolism to regulation of ploidy/nucleation and repair in the heart. 


Grant ID