Proteins that control the geometry of ciliary ends
journal contributionposted on 25.03.2019 by Panagiota Louka, Krishna Kumar Vasudevan, Mayukh Guha, Ewa Joachimiak, Dorota Wloga, Raphaël F.‑X. Tomasi, Charles N. Baroud, Pascale Dupuis‑Williams, Domenico F. Galati, Chad G. Pearson, Luke M. Rice, James J. Moresco, Yu‑Yang Jiang, Karl Lechtreck, William Dentler, Jacek Gaertig, John R. Yates III
Any type of content formally published in an academic journal, usually following a peer-review process.
We studied three conserved ciliary proteins, FAP256/CEP104, CHE12/Crescerin and ARMC9, in the ciliate Tetrahymena. Using a combination of super-resolution structural illumination and electron microscopy we show that these proteins localize near the plus ends of ciliary microtubules where they control their length and subsequently the structural organization of ciliary ends. Our results show that defects in the geometry of ciliary ends affect the function of cilia.