Measuring Cardiomyocyte Contractility and Calcium Handling In Vitro.
journal contributionposted on 06.03.2019 by Przemek A. Gorski, changwon kho, Jae Gyun Oh
Any type of content formally published in an academic journal, usually following a peer-review process.
In vitro measurements of cardiomyocyte contractility and Ca2+ handling have been used as a platform for
determining physiological consequence of various genetic manipulations and identifying potential therapeutic
targets for the treatment of heart failure. The Myocyte Calcium and Contractility System (IonOptix)
offers a simultaneous trace of sarcomere movements and changes of intracellular Ca2+ levels in a single
cardiomyocyte. Herein, we describe a modified protocol for the isolation of adult cardiomyocytes from
murine hearts and provide a step-by-step description on how to analyze cardiomyocyte Ca2+ transient and
contractility data collected using the IonOptix system. In our modified protocol, we recommend a novel
cannulation technique which simplifies this difficult method and leads to improved viability of isolated
cardiomyocytes. In addition, a comprehensive analysis of intracellular Ca2+ handling, SR Ca2+ load,
myofilament Ca2+ sensitivity, and cardiomyocyte contractility is described in order to provide important
insights into myocardial mechanics.