10.25376/hra.8171144.v1
Yang Liu
Yang
Liu
Wenyan Fu
Wenyan
Fu
Kendall Seese
Kendall
Seese
Amelia Yin
Amelia
Yin
Hang Yin
Hang
Yin
Ectopic brown adipose tissue formation within skeletal muscle after brown adipose progenitor cell transplant augments energy expenditure.pdf
Health Research Alliance
2019
transplantation
Engraftment
thermogenesis
UCP1
Obesity
diabetes and endocrinology/obesity
Animal Physiology - Systems
Cell Development, Proliferation and Death
Cell Metabolism
Molecular Biology
Physiology
Biomedical Engineering not elsewhere classified
Metabolic Medicine
2019-05-22 20:12:50
Journal contribution
https://hra.figshare.com/articles/journal_contribution/Ectopic_brown_adipose_tissue_formation_within_skeletal_muscle_after_brown_adipose_progenitor_cell_transplant_augments_energy_expenditure_pdf/8171144
Brown adipose tissue (BAT) thermogenesis increases energy expenditure (EE). Expanding the volume of
active BAT via transplantation holds promise as a therapeutic strategy for morbid obesity and diabetes. Brown
adipose progenitor cells (BAPCs) can be isolated and expanded to generate autologous brown adipocyte implants.
However, the transplantation of brown adipocytes is currently impeded by poor efficiency of BAT tissue formation
in vivo and undesirably short engraftment time. In this study, we demonstrated that transplanting BAPCs into limb
skeletal muscles consistently led to the ectopic formation of uncoupling protein 1 (UCP1)+pos adipose tissue with
long-term engraftment (>4 mo). Combining VEGF with the BAPC transplant further improved BAT formation in
muscle. Ectopic engraftment ofBAPC-derivedBAT in skeletalmuscle augmented the EE of recipientmice. Although
UCP1 expression declined in long-term BAT grafts, this deterioration can be reversed by swimming exercise because
of sympathetic activation. This study suggests that intramuscular transplantation of BAPCs represents a promising
approach to derive functional BAT engraftment, which may be applied to therapeutic BAT transplantation and
tissue engineering.